Celer Series 293 Cell Serum-free Medium Facilitates The Efficient Production Of Adenovirus Vector Vaccines

Celer series 293 cell serum-free medium

Product features:

1. No serum, no protein, no animal source

2. Suitable for HEK293, 293T, 293F and other 293 cells

3. Support the rapid suspension acclimation and high-density culture of 293 cells

4. Design a variety of special media products for different application scenarios such as adenovirus amplification, protein expression and virus packaging

Product advantages:

1. Large-scale production of adenovirus vector-based vaccines can reach a yield of 10¹⁰ TCID₅₀/mL

(2) Efficient packaging of viral vectors, the production of AAV and lentivirus could reach 10¹¹vg/mL and 10⁷ TU/mL, respectively

3. High protein expression, transient protein expression can reach 900mg/L in the flow process (day 8)

4. Advanced GMP production process to ensure excellent product batch consistency (Cpk≥1.33, RSD<5%)*

5. Have ISO13485:2016 and MDSAP double quality system certification, production in line with GMP specifications

6. Provide complete supporting documents to make your application process easier

7. It has served several clinical projects, and has been successfully applied in the production project of 1000 upgrade reactor

8. A technical support team with rich project experience can provide comprehensive cell culture technical support and services

* Key quality attribute Cpk≥1.33, Cpk is an internationally accepted index to characterize the process capability, Cpk≥1.33 indicates that the process is well controlled and the product batch variation is small. PPM level mixing uniformity < 5%, RSD refers to the relative standard deviation, and lower RSD values indicate less variability in the production process.

Product performance:

Adenovirus vector production

Celer series medium supported efficient amplification of adenovirus, and the virus yield could reach 10¹⁰TCID₅₀/mL. It has achieved the production scale of thousands of upgraded reactors, and has served customer projects for clinical application.

Packaging of virus

The Celer series medium was used for virus packaging, which is simple to perform and easy to scale up. The culture medium supported AAV packaging with a yield of 10¹¹vg/mL. Support LV packaging, production up to 10⁷TU/mL.

Protein expression

The use of Celer series media supports high protein expression, with expression levels of up to 100 mg/liter, and is also easy to process and scale up.