Vigor Series Insect Cell Serum-free Medium, Recombinant Protein Vaccine Production Is Preferred

Vigor-S101S Serum-free medium for insect cells

Product features:

1. No serum, no animal source, no protein components

2. Support high density insect cell culture and high efficiency protein expression based on BEVS

3. It can be applied to Sf9 and High five cells simultaneously

4. Support subunit or VLP vaccine production, recombinant protein expression, and rAAV production

Product advantages:
1. It has been applied in the research and development and production projects of multiple subunit vaccines and VLP vaccines, and the training effect has stood out among many competing products
2. Advanced CMPM production process to ensure excellent batch consistency (Cpk≥1.33, RSD<5%)*
3. Provide complete supporting documents to make your application process easier
4. Has ISO13485:2016 and MDSAP double quality system certification, production in line with GMP specifications
5. The technical support team has a deep professional background and rich project experience, to provide customers with professional and comprehensive solutions

* Key quality attribute Cpk≥1.33, Cpk is an internationally accepted index to characterize the process capability, Cpk≥1.33 indicates that the process is well controlled and the product batch variation is small. PPM level mixing uniformity < 5%, RSD refers to the relative standard deviation, and lower RSD values indicate less variability in the production process.

Product performance:
Cells were seeded at 1×10⁶cells/mL in Vigor-S101S medium and subcultured every 48 hours. The density of Sf9 cells reached 3-5×10⁶cells/mL (doubling time 21-30 hours), and the density of High five cells reached 5-7×10⁶cells/mL (doubling time 17-21 hours), which could be stably passaged for a long time.

The maximum density of Sf9 cells in Vigor-S101S insect cell serum-free medium was 12-14×10⁶cells/mL. Compared with the well-known imported medium, Sf9 cells grew faster, had higher density, and maintained for a longer time. High five cells can reach a maximum density of 9-11×10⁶cells/mL in Vigor-S101S insect cell serum-free medium, which is superior to the well-known imported medium that specifically cultivates High five at a consistent level.

In Vigor-S101S insect cell serum-free medium, Sf9 cells were used to produce the seed toxin, and the titer was better than that of similar products on the market.

In Vigor-S101S insect cell serum-free medium, the use of High five cells to express protein yield has advantages.