MDCK Cell Culture Medium

A:All of our powder media products are packaged in aluminum foil bags. The aluminum foil bags provide excellent light protection, low water permeability, and low oxygen permeability, effectively preventing the deterioration of powdered culture media due to light exposure, humidity, and oxidation. You can view product images on the respective product pages.

A: This product is highly hygroscopic. It is recommended to use it immediately after opening. If further storage is necessary, strict sealing methods such as heat sealing or using sealing clips should be employed to seal tightly and prevent moisture absorption that may lead to product failure.

A: The Eden series CHO culture media have a shelf life of 1 to 2 years, validated through real-time stability experiments and accelerated testing.

A: Currently, there are no quality-adjusting agent products available. However, we can provide some experimental protocols based on customer data to assist them in antibody/protein quality modulation.

A: The Eden series CHO culture media do not contain hydrolysates, insulin, cytokines, glutamine, antibiotics, HEPES, phenol red, and HT (Hypoxanthine and Thymidine). If your cell line is dependent on cytokines, hydrolysates, glutamine, or nucleotides (such as hypoxanthine and thymidine), it is recommended to add appropriate concentrations of these substances to the Eden series culture media.

A: CHO media refers to cell culture media specifically designed for the growth and maintenance of Chinese hamster ovary (CHO) cells, which are commonly used in biotechnology and pharmaceutical industries to produce therapeutic proteins and monoclonal antibodies. CHO media are typically composed of a variety of nutrients, growth factors, and supplements that promote cell growth, proliferation, and protein expression in these cells. There are several types of CHO media available with different formulations optimized for specific applications, including serum-free and chemically defined options. The Eden series CHO culture media provided by BioEngine are chemically defined media that are serum-free, protein-free, and animal-derived components free.

A:DMEM and RPMI are two commonly used types of media for cell culture, and they have some differences in their composition and usage. Here are some of the key differences:

 

1. Nutrient composition: DMEM (Dulbecco's Modified Eagle's Medium) and RPMI (Roswell Park Memorial Institute) have different nutrient compositions. DMEM contains higher levels of glucose, amino acids, vitamins, and sodium pyruvate, while RPMI has a lower glucose concentration and a different amino acid and vitamin composition.

2. pH: DMEM has a higher pH (7.4-7.6) compared to RPMI (7.2-7.4).

3. Usage: DMEM is a more general-purpose medium and can be used for a wide range of cell types, including adherent and non-adherent cells, while RPMI is typically used for the culture of immune cells such as lymphocytes and hybridomas.

4. Serum requirement: RPMI is often used with lower serum concentrations (e.g. 5-10%) than DMEM, which may require higher serum concentrations (e.g. 10-20%).

Overall, the choice between DMEM and RPMI depends on the specific cell type being cultured and the experimental conditions.

A:Fed-batch culture is a type of bioreactor operation used in industrial biotechnology to cultivate microorganisms or cells for the production of various bioproducts, such as vaccines, enzymes, and biofuels. In a fed-batch culture, a small initial volume of culture medium is used to inoculate a bioreactor, which is then gradually supplemented with additional nutrients, such as sugars, amino acids, and vitamins, over the course of the culture process.

 

The goal of a fed-batch culture is to maximize cell growth and productivity while maintaining a stable culture environment. By controlling the rate and timing of nutrient addition, the culture can be kept in a state of controlled growth, avoiding the depletion of nutrients and accumulation of waste products that can limit growth and product formation in batch cultures. Additionally, the use of a fed-batch culture can allow for the accumulation of high cell densities and the optimization of production conditions, leading to higher yields and greater efficiency in bioprocesses.

A:Suspension cell culture is a type of cell culture where cells are grown in a liquid medium and are suspended in the culture medium, as opposed to being attached to a surface or substrate. Some of the advantages of suspension cell culture are:

 

1. Scalability: Suspension cell cultures can be easily scaled up to produce large quantities of cells. This makes them particularly useful for biomanufacturing and the production of recombinant proteins.

2. Flexibility: Suspension cultures can be adapted to a wide range of culture conditions, such as pH, temperature, and nutrient availability. This allows for the optimization of cell growth and productivity.

3. Homogeneity: Suspension cultures provide a more homogeneous population of cells than adherent cultures, where cells may exhibit varying degrees of differentiation and proliferation.

4. Reduced risk of contamination: Suspension cultures are less prone to contamination by bacteria or fungi than adherent cultures, as there are no surfaces for microorganisms to adhere to.

5. Ease of harvesting: Cells in suspension culture can be easily harvested using centrifugation, filtration, or other methods. This simplifies downstream processing and reduces the risk of damage to the cells.

 

Overall, suspension cell culture offers several advantages over other types of cell culture, particularly in the context of large-scale biomanufacturing and the production of recombinant proteins.

A:"Gene therapy is a technique that modifies a person's genes to treat or cure disease 1. Gene therapy can work by several mechanisms, such as:

●Replacing a disease-causing gene with a healthy copy of the gene.

●Inactivating or deleting a disease-causing gene.

●Introducing a new or modified gene to help fight disease.

 

Gene therapy is a promising and innovative field of medicine that has potential applications for many diseases, such as cancer, genetic disorders, infectious diseases, and autoimmune diseases 23. However, gene therapy also faces many challenges and risks, such as safety, efficacy, ethical issues, and regulatory hurdles.

Gene therapy usually be delivered to the cells by virus vector, such as AAV, Adv and RV. BioEngine provides vigor series insect cell media for large scale AAV production."

A: NO. BioEngine provides various serum-free media products and cell culture services.